Hsp70通过cAMP/PKA信号通路抑制猪肌内前体脂肪细胞成脂分化的研究

doi:10.16174/j.issn.1673-4645.2026.05.010

Abstract

Abstract: To investigate the role and underlying mechanism of heat shock protein 70 (Hsp70) in the adipogenic differentiation of porcine intramuscular preadipocytes, intramuscular preadipocytes isolated from the longissimus dorsi muscle of 3-day-old piglets were used in this study. An adipogenic induction culture system was established, and the dynamic expression patterns of Hsp70 and lipid metabolism-related genes were examined on days 0, 2, 4, and 6 of induction. Meanwhile, a recombinant adenovirus-mediated Hsp70 overexpression model was constructed to analyze its effects on adipogenic differentiation, lipid metabolism-related genes, and the cAMP/PKA/PPARγ signaling pathway. The results showed that Hsp70 expression initially increased and subsequently decreased during adipogenic differentiation, reaching a peak on day 4 of induction. Its expression pattern was partially synchronous with those of AP2 and LPL, suggesting that Hsp70 may participate in the transition between stages of adipogenic differentiation and the remodeling of lipid metabolism. Hsp70 overexpression significantly or highly significantly decreased the mRNA expression levels of PPARγ, C/EBPα, and FAS, reduced lipid droplet accumulation, and significantly increased LPL expression, indicating that Hsp70 inhibits the adipogenic differentiation of porcine intramuscular preadipocytes. Further investigation revealed that Hsp70 overexpression significantly reduced intracellular cAMP levels, inhibited PKA phosphorylation and activation, and downregulated PPARγ protein expression. In conclusion, Hsp70 negatively regulates the adipogenic differentiation of porcine intramuscular preadipocytes, and this effect may be mediated by inhibition of the cAMP/PKA/PPARγ signaling axis. These findings provided experimental evidence for elucidating the molecular mechanisms underlying intramuscular fat deposition in pigs.

Key words: Hsp70, pig, porcine intramuscular preadipocytes, adipogenic differentiation, cAMP/PKA/PPARγ signaling pathway

CLC Number:

S828

CHU Han, DENG Shifan, CHU Ruige, LV Zhidong, MA Jianing1 YU Chunmei, CAO Jingxuan, LI Wenjun, PU Lei. The research of Hsp70 on inhibiting adipogenic differentiation of porcine intramuscular preadipocytes through cAMP/PKA signaling pathway[J].China Swine Industry, 2026, 21(5): 41-48.

References

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The research of Hsp70 on inhibiting adipogenic differentiation of porcine intramuscular preadipocytes through cAMP/PKA signaling pathway

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