巴马香猪FGF2基因克隆、生物信息学分析和组织表达研究

doi:10.16174/j.issn.1673-4645.2026.05.008

Abstract

Abstract: This study aimed to explore the biological function of fibroblast growth factor 2 (FGF2) in the mammary gland development of Bama Xiang pigs, clarify its sequence characteristics, encoded protein properties and tissue expression pattern, so as to provide basic data for subsequent functional verification. Specific primers were designed with reference to the reference sequence of porcine FGF2 gene (GenBank accession No.: NM_001405514.1) from NCBI database. The CDS region of FGF2 gene was cloned by RT-PCR using cDNA from mammary gland tissue of Bama Xiang pigs as the template. Sequence alignment, phylogenetic tree construction, and structural and functional analysis of the encoded protein were performed using bioinformatics software. The expression characteristics of FGF2 gene in different tissues of Bama Xiang pigs were detected by qPCR. The CDS sequence of FGF2 gene from Bama Xiang pigs was successfully cloned, with a full length of 468 bp, encoding 155 amino acids. The sequence shared 98.7% homology with that of domestic pigs, was highly conserved in mammals, and had the closest genetic relationship with domestic pigs in evolution. The encoded protein was an alkaline, stable and hydrophilic protein with a molecular weight of 17.25 kDa, without signal peptide and transmembrane domain. It had multiple potential phosphorylation modification sites, and could interact with FGFR family proteins and key regulatory factors of mammary gland development. FGF2 gene showed a ubiquitous expression pattern in the detected tissues, with significantly higher expression levels in the mammary gland and ovary than those in other tissues (P＜0.05), and the lowest expression level in the liver. This study clarified the sequence evolution, protein structure and tissue expression pattern of FGF2 gene in Bama Xiang pigs, which provided a theoretical and experimental basis for further exploring its molecular regulatory mechanism in mammary gland development.

Key words: Bama Xiang pig, FGF2, gene cloning, bioinformatics analysis, mammary gland development

CLC Number:

S828.8

LIU Cong, FU Zhangna, HE Yiyun, WU Xiaoxiao, LIANG Shuang, ZHANG Yanping, LIANG Jing. Cloning, bioinformatics analysis and tissue expression study of FGF2 gene in Guangxi Bama mini-pigs[J].China Swine Industry, 2026, 21(5): 23-32.

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Cloning, bioinformatics analysis and tissue expression study of FGF2 gene in Guangxi Bama mini-pigs

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