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China Swine Industry ›› 2026, Vol. 21 ›› Issue (5): 49-57.doi: 10.16174/j.issn.1673-4645.2026.05.012

• Genetic Breeding • Previous Articles     Next Articles

Effect of SNPs in intron 1 of the PTN gene on reproductive traits in Kele pigs

ZHAO Zhilin1, SHENG Yingxia2, JIANG Xueqin3, QU Yugong1, WANG Zhen4*, ZHANG Yiyu5*   

  1. 1Qingzhou Animal Husbandry Development Center, Qingzhou 262500, China; 2Shandong Provincial Animal Husbandry Station, Jinan 250109, China; 3Yuli Town Animal Husbandry and Veterinary Station, Rushan 264517, China; 4Guizhou University Tea College, Guiyang 550025, China; 5Key Laboratory of Plateau and Mountain Animal Genetics, Breeding and Reproduction of the Ministry of Education / Guizhou University Xiang Pig Research Institute, Guizhou University, Guiyang 550025, China
  • Online:2026-11-13 Published:2026-10-25

Abstract: To identify the SNP of the PTN gene in Kele pigs, analyze their population genetic characteristics, linkage disequilibrium and haplotype/ diplotype distribution, and explore their correlation with reproductive traits, thereby providing clues for candidate gene mining of reproductive traits in Kele pigs. A total of 116 Kele multiparous pigs were used as experimental materials. Six pairs of primers were designed for PCR amplification; effective primers were screened and the amplification products were sequenced and aligned to identify SNP loci. Population genetic parameters such as allele frequency, genotype frequency and polymorphism information content (PIC) were calculated, Hardy-Weinberg equilibrium was tested and linkage disequilibrium among loci and haplotype/ diplotype distribution were analyzed. A general linear model was used to analyze the association of SNP loci and diplotypes with reproductive traits of Kele pigs. Four SNP loci (g.12234927 C>T, g.12235085 C>T, g.12235089 C>T and g.12235093 C>T) were identified, all located within the F1/R1 amplified region of intron 1 of the PTN gene. The most frequent allele and genotype of the four loci were C and CC, respectively, with PIC ranging from 0.274 to 0.364, indicating a moderate polymorphism. After the Bonferroni correction, the genotype distribution of all loci did not deviate from the Hardy-Weinberg equilibrium. g.12234927 C>T and g.12235089 C>T were completely linked and showed strong linkage disequilibrium with g.12235085 C>T. Four haplotypes and ten diplotypes were detected, with the most frequent haplotype H1 and diplotype H1H2 at frequencies of 0.345 and 0.172, respectively. Within this sample, g.12234927 C>T and g.12235089 C>T were statistically associated with total number born and individual birth weight, and some diplotypes were associated with the number of weaned piglets and individual birth weight; however, only 8 individuals carried the TT genotype, and the stability of the effect estimates remains to be verified. In conclusion, the first intron of the PTN gene in Kele pigs was a candidate variation region for reproductive traits. The results of this study represented exploratory findings of candidate variations and require further validation with enlarged populations and multiple-comparison correction before their application value can be assessed.

Key words: Kele pig, pleiotrophin(PTN), single nucleotide polymorphisms (SNPs), reproductive traits, intron

CLC Number: 

  • S828
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