柯乐猪PTN基因第1内含子SNPs对繁殖性状的影响

doi:10.16174/j.issn.1673-4645.2026.05.012

摘要/Abstract

摘要： 对柯乐猪PTN基因SNP位点进行鉴定，分析其群体遗传特征、连锁不平衡及单双倍型分布，并探究其与繁殖性状的相关性，为柯乐猪繁殖性状的候选基因挖掘提供线索。以116头经产柯乐猪为试验材料，设计6对引物开展PCR扩增，筛选有效引物并对扩增产物测序比对，鉴定SNPs变异位点；计算各SNPs位点等位基因频率、基因型频率、多态信息含量等群体遗传参数，检验Hardy-Weinberg平衡状态，分析位点间连锁不平衡程度及单双倍型分布；采用一般线性模型分析SNPs位点及双倍型与柯乐猪繁殖性状的关联。4个SNPs位点（g.12234927C＞T、g.12235085C＞T、g.12235089C＞T、g.12235093C＞T）均位于PTN基因第1内含子F1/R1扩增区段；4个位点频率最高等位基因与基因型均为C和CC，多态信息含量介于0.274～0.364之间，属中度多态；经Bonferroni校正后，各位点基因型分布均未偏离Hardy-Weinberg平衡；g.12234927C＞T与g.12235089C＞T完全连锁，且与g.12235085C＞T呈强连锁不平衡；共检出4种单倍型和10种双倍型，频率最高的单倍型H1和双倍型H1H2频率分别为0.345和0.172。在本试验样本内，g.12234927C＞T和g.12235089C＞T位点与总产仔数、初生个体重存在统计学关联，部分双倍型与断奶仔猪数、初生个体重相关，其中TT基因型个体仅8头，效应估计的稳定性有待验证。综上，柯乐猪PTN基因第1内含子为繁殖性状的候选变异区段，本研究结果为候选变异的探索性发现，需经扩大群体和多重比较校正等进一步验证后，方可评估其应用价值。

关键词: 柯乐猪, PTN, SNPs, 繁殖性, 内含子

Abstract: To identify the SNP of the PTN gene in Kele pigs, analyze their population genetic characteristics, linkage disequilibrium and haplotype/ diplotype distribution, and explore their correlation with reproductive traits, thereby providing clues for candidate gene mining of reproductive traits in Kele pigs. A total of 116 Kele multiparous pigs were used as experimental materials. Six pairs of primers were designed for PCR amplification; effective primers were screened and the amplification products were sequenced and aligned to identify SNP loci. Population genetic parameters such as allele frequency, genotype frequency and polymorphism information content (PIC) were calculated, Hardy-Weinberg equilibrium was tested and linkage disequilibrium among loci and haplotype/ diplotype distribution were analyzed. A general linear model was used to analyze the association of SNP loci and diplotypes with reproductive traits of Kele pigs. Four SNP loci (g.12234927 C＞T, g.12235085 C＞T, g.12235089 C＞T and g.12235093 C＞T) were identified, all located within the F1/R1 amplified region of intron 1 of the PTN gene. The most frequent allele and genotype of the four loci were C and CC, respectively, with PIC ranging from 0.274 to 0.364, indicating a moderate polymorphism. After the Bonferroni correction, the genotype distribution of all loci did not deviate from the Hardy-Weinberg equilibrium. g.12234927 C＞T and g.12235089 C＞T were completely linked and showed strong linkage disequilibrium with g.12235085 C＞T. Four haplotypes and ten diplotypes were detected, with the most frequent haplotype H1 and diplotype H1H2 at frequencies of 0.345 and 0.172, respectively. Within this sample, g.12234927 C＞T and g.12235089 C＞T were statistically associated with total number born and individual birth weight, and some diplotypes were associated with the number of weaned piglets and individual birth weight; however, only 8 individuals carried the TT genotype, and the stability of the effect estimates remains to be verified. In conclusion, the first intron of the PTN gene in Kele pigs was a candidate variation region for reproductive traits. The results of this study represented exploratory findings of candidate variations and require further validation with enlarged populations and multiple-comparison correction before their application value can be assessed.

Key words: Kele pig, pleiotrophin(PTN), single nucleotide polymorphisms (SNPs), reproductive traits, intron

中图分类号: S828；S814

赵志林, 盛英霞, 姜雪芹, 曲玉功, 王振, 张依裕. 柯乐猪PTN基因第1内含子SNPs对繁殖性状的影响[J]. 中国猪业, 2026, 21(5): 49-57.

ZHAO Zhilin, SHENG Yingxia, JIANG Xueqin, QU Yugong, WANG Zhen, ZHANG Yiyu. Effect of SNPs in intron 1 of the PTN gene on reproductive traits in Kele pigs[J]. China Swine Industry, 2026, 21(5): 49-57.

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