Hsp70通过cAMP/PKA信号通路抑制猪肌内前体脂肪细胞成脂分化的研究

doi:10.16174/j.issn.1673-4645.2026.05.010

摘要/Abstract

摘要： 为探究热休克蛋白70（Hsp70）在猪肌内前体脂肪细胞成脂分化中的作用及其机制，本研究以3日龄仔猪背最长肌分离获得的猪肌内前体脂肪细胞为材料，采用成脂诱导培养体系，分别于诱导0、2、4和6d检测Hsp70及脂质代谢相关基因的动态表达；同时利用重组腺病毒构建Hsp70过表达模型，分析其对成脂分化、脂质代谢相关基因及cAMP/PKA/PPARγ信号通路的影响。结果显示，Hsp70在成脂分化过程中呈先升高后降低的表达趋势，并于诱导第4d达到峰值，其表达变化与AP2和LPL等基因具有一定同步性，提示Hsp70可能参与成脂分化阶段转换及脂质代谢重塑。Hsp70过表达后，PPARγ、C/EBPα和FAS的mRNA表达显著或极显著下降，脂滴积聚减少，而LPL表达显著升高，表明Hsp70对猪肌内前体脂肪细胞成脂分化具有抑制作用。进一步研究发现，Hsp70过表达显著降低细胞内cAMP水平，抑制PKA磷酸化激活，并下调PPARγ蛋白表达。综上，Hsp69在猪肌内前体脂肪细胞成脂分化中发挥负向调控作用，其机制可能与抑制cAMP/PKA/PPARγ信号轴有关。本研究为阐明猪肌内脂肪沉积的分子调控机制提供了试验依据。

关键词: Hsp70, 猪, 肌内前体脂肪细胞, 成脂分化, cAMP/PKA/PPARγ信号通路

Abstract: To investigate the role and underlying mechanism of heat shock protein 70 (Hsp70) in the adipogenic differentiation of porcine intramuscular preadipocytes, intramuscular preadipocytes isolated from the longissimus dorsi muscle of 3-day-old piglets were used in this study. An adipogenic induction culture system was established, and the dynamic expression patterns of Hsp70 and lipid metabolism-related genes were examined on days 0, 2, 4, and 6 of induction. Meanwhile, a recombinant adenovirus-mediated Hsp70 overexpression model was constructed to analyze its effects on adipogenic differentiation, lipid metabolism-related genes, and the cAMP/PKA/PPARγ signaling pathway. The results showed that Hsp70 expression initially increased and subsequently decreased during adipogenic differentiation, reaching a peak on day 4 of induction. Its expression pattern was partially synchronous with those of AP2 and LPL, suggesting that Hsp70 may participate in the transition between stages of adipogenic differentiation and the remodeling of lipid metabolism. Hsp70 overexpression significantly or highly significantly decreased the mRNA expression levels of PPARγ, C/EBPα, and FAS, reduced lipid droplet accumulation, and significantly increased LPL expression, indicating that Hsp70 inhibits the adipogenic differentiation of porcine intramuscular preadipocytes. Further investigation revealed that Hsp70 overexpression significantly reduced intracellular cAMP levels, inhibited PKA phosphorylation and activation, and downregulated PPARγ protein expression. In conclusion, Hsp70 negatively regulates the adipogenic differentiation of porcine intramuscular preadipocytes, and this effect may be mediated by inhibition of the cAMP/PKA/PPARγ signaling axis. These findings provided experimental evidence for elucidating the molecular mechanisms underlying intramuscular fat deposition in pigs.

Key words: Hsp70, pig, porcine intramuscular preadipocytes, adipogenic differentiation, cAMP/PKA/PPARγ signaling pathway

中图分类号: S828；S813.3

楚菡, 邓诗凡, 楚蕊鸽, 吕志东, 马嘉宁, 于春梅, 曹靖轩, 李文军, 蒲蕾. Hsp70通过cAMP/PKA信号通路抑制猪肌内前体脂肪细胞成脂分化的研究[J]. 中国猪业, 2026, 21(5): 41-48.

CHU Han, DENG Shifan, CHU Ruige, LV Zhidong, MA Jianing1 YU Chunmei, CAO Jingxuan, LI Wenjun, PU Lei. The research of Hsp70 on inhibiting adipogenic differentiation of porcine intramuscular preadipocytes through cAMP/PKA signaling pathway[J]. China Swine Industry, 2026, 21(5): 41-48.

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