藏香猪流行性腹泻病毒的PCR检测及毒株分型

doi:10.16174/j.issn.1673-4645.2024.02.008

摘要/Abstract

摘要： 为明确四川省凉山彝族自治州某藏香猪规模化养殖场哺乳仔猪腹泻的病因，试验采集腹泻仔猪的粪便和肠道样品进行实验室病原学诊断，采用RT-PCR 方法检测4 种常见肠道病毒的感染情况。检测结果显示，猪流行性腹泻病病毒（PEDV）检测为阳性，猪delta 冠状病毒、猪传染性胃肠炎病毒和猪A 群轮状病毒3 种病毒均为阴性。进一步采用RT-PCR 扩增该毒株S1 基因序列并测序。结果显示，该毒株与G2 型变异毒株同源性最高，与其他基因型毒株同源性相对较低，因此判定该猪场仔猪发病是由猪流行性腹泻病毒G2 型变异毒株感染所致。对病猪进行紧急免疫接种、治疗、消毒等综合防控措施，控制住了疫病的蔓延和发展。总结此次猪流行性腹泻病的治疗经验，为养猪场对该疫病的治疗和防控提供借鉴。

中图分类号: S828；S852.65

阳红莲，何玉华，朱玲，徐志文. 藏香猪流行性腹泻病毒的PCR检测及毒株分型[J]. 中国猪业, 2024, 19(2): 67-73.

参考文献

[1] MCGOLDRICK A, LOWINGS JP, PATON DJ. Characterisation of a recent virulent transmissible gastroenteritis virus from Britain with a deleted ORF 3a[J]. Archives of Virology, 1999, 144(4):763-770. [2] KIM L, CHANG KO, SESTAK K, et al. Development of a reverse transcription-nested polymerase chain reaction assay for differential diagnosis of transmissible gastroenteritis virus and porcine respiratory coronavirus from feces and nasal swabs of infected pigs[J]. Journal of Veterinary Diagnostic Investigation, 2000, 12(4):385-388. [3] ZHANG F, LUO S, GU J, et al. Prevalence and phylogenetic analysis of porcine diarrhea associated viruses in southern China from 2012 to 2018[J]. BMC Veterinary Research, 2019, 15(1):470. [4] GUO R, FAN B, CHANG X, et al. Characterization and evaluation of the pathogenicity of a natural recombinant transmissible gastroenteritis virus in China[J]. Virology, 2020, 545:24-32. [5] WANG D, FANG L, XIAO S. Porcine epidemic diarrhea in China[J]. Virus Research, 2016, 226:7-13. [6] HAVE P, MOVING V, SVANSSON V, et al. Coronavirus infection in mink (Mustela vison). Serological evidence of infection with a coronavirus related to transmissible gastroenteritis virus and porcine epidemic diarrhea virus[J]. Veterinary Microbiology, 1992, 31(1):1-10. [7] PENSAERT MB, DE BOUCK P. A new coronavirus-like particle associated with diarrhea in swine[J]. Archives of Virology, 1978, 58(3):243-247. [8] LEE C. Erratum to: porcine epidemic diarrhea virus: an emerging and re-emerging epizootic swine virus[J]. Virology Journal, 2016, 12:193-208. [9] 左媛媛, 徐天刚, 戈胜强, 等. 我国猪流行性腹泻病毒流行现状及分子遗传演化特征[J]. 中国动物检疫, 2022, 39(9):9-17. ZUO YY, XU TG, GE SQ, et al. Prevalence and molecular genetic evolution of porcine epidemic diarrhea virus in China[J]. China Animal Health Inspection, 2022, 39(9):9-17. [10] WANG L, BYRUM B, ZHANG Y. Detection and genetic characterization of deltacoronavirus in pigs, Ohio, USA, 2014[J]. Emerging Infectious Diseases, 2014, 20(7):1227-1230. [11] WANG L, HAYES J, BYRUM B, et al. US variant porcine epidemic diarrhea virus: histological lesions and genetic characterization [J]. Virus Genes, 2016, 52(4):578-581. [12] 贾荣玲, 刘耀东, 路鑫. 猪德尔塔冠状病毒病的诊断与防治[J]. 河南农业, 2018(33):47-48. JIA RL, LIU YD, LU X. Diagnosis and prevention of porcine delta coronavirus disease[J]. Agriculture of Henan, 2018(33):47-48. [13] 宋亚兵, 徐帅飞, 苏丹萍, 等. 2012年—2016年广东省猪丁型冠状病毒的流行病学调查及分析[J]. 中国预防兽医学报, 2018, 40(10):886-890. SONG YB, XU SF, SU DP, et al. Epidemiological survey and analysis of porcine deltacoronavirus in Guangdong province from 2012 to 2016[J]. Chinese Journal of Preventive Veterinary Medicine, 2018, 40(10):886-890. [14] WOO PC, LAU SK, LAM CS, et al. Discovery of seven novel mammalian and avian coronaviruses in the genus deltacoronavirus supports bat coronaviruses as the gene source of alphacoronavirus and betacoronavirus and avian coronaviruses as the gene source of gammacoronavirus and deltacoronavirus[J]. Journal of Virology, 2012, 86(7):3995-4008. [15] JANG G, LEE KK, KIM SH, et al. Prevalence, complete genome sequencing and phylogenetic analysis of porcine deltacoronavirus in South Korea, 2014-2016[J]. Transboundary and Emerging Diseases, 2017, 64(5):1364-1370. [16] LORSIRIGOOL A, SAENG-CHUTO K, TEMEEYASEN G, et al. The first detection and full-length genome sequence of porcine deltacoronavirus isolated in Lao PDR[J]. Archives of Virology, 2016, 161(10):2909-2911. [17] 闫晓光, 丁庆文, 任豪杰, 等. 猪主要腹泻病毒多重RT-PCR检测方法的建立及初步应用[J]. 中国兽医学报, 2023, 43(4):654-659,667. YAN XG, DING QW, REN HJ, et al. Establishment and preliminary application of multiplex RT-PCR method for detection of major porcine diarrhea ciruses[J]. Chinese Journal of Veterinary Science, 2023, 43(4):654-659,667. [18] 任玉鹏, 陈弟诗, 苏生禹, 等. 三种猪腹泻病毒性病原多重RT-PCR检测方法的建立及应用[J]. 中国兽医科学, 2013, 43(10):1047-1051. REN YP, CHEN DS, SU SY, et al. Development and application of triplex RT-PCR for detection of the three pathogens of viral diarrhea in pigs[J]. Chinese Veterinary Science, 2013, 43(10):1047-1051. [19] 崔建涛, 韩昊莹, 党家刚, 等. 猪流行性腹泻病毒流行株S全基因的遗传进化与重组分析[J]. 中国兽医学报, 2020, 40(9):1671-1676. CUI JT, HAN HY, DANG JG, et al. Genetic and recombinat analysis of S gene of porcine epidemic diarrhea virus[J]. Chinese Journal of Veterinary Science, 2020, 40(9):1671-1676. [20] 王英贺, 姚新华, 苑淑贤, 等. 猪常见病毒性腹泻的特点及预防措施[J]. 动物医学进展, 2015, 36(11):110-114. WANG YH, YAO XH, YUAN SX, et al. Characteristics and countermeasures of common porcine virus diarrhea[J]. Progress in Veterinary Medicine, 2015, 36(11):110-114. [21] 仝帅. 2016-2018年山东省部分地区猪病毒性腹泻的感染情况调查[D]. 泰安: 山东农业大学, 2019. TONG S. Epidemiological investigation of porcine viral diarrhea in parts of Shandong province in 2016 to 2018[D], Taian: Shandong Agricultural University, 2019. [22] 赵曦晨, 殷舒, 邓近平, 等. 藏香猪的生物学特性及饲养管理研究进展[J]. 饲料博览, 2019(5):26-30. ZHAO XC, YIN S, DENG JP, et al. Research progress on biological characteristics and feeding management of the Zang scent pigs[J]. Feed Review, 2019(5):26-30. [23] SUN Q, CAI RJ, CHEN YQ, et al. Outbreak of porcine epidemic diarrhea in suckling piglets, China[J]. Emerging Infectious Diseases, 2012, 18(1):161-163. [24] TIAN Y, YU Z, CHENG K, et al. Molecular characterization and phylogenetic analysis of new variants of the porcine epidemic diarrhea virus in Gansu, China in 2012[J]. Viruses, 2013, 5(8):1991-2004. [25] 王栓群, 孙瑞芹, 刘燕玲, 等. 华南地区猪流行性腹泻病毒的分子流行病学调查与分析[J]. 广东农业科学, 2015, 42(6):97-104. WANG SQ, SUN RQ, LIU YL, et al. Investigation and analysis on molecular epidemiology of porcine epidemic diarrhea virus in south China[J]. Guangdong Agricultural Sciences, 2015, 42(6):97-104. [26] 郑逢梅, 霍金耀, 赵军, 等. 2010—2012年华中地区猪流行性腹泻病毒分子特征和遗传进化分析[J]. 病毒学报, 2013, 29(2):197-205. ZHENG FM, HUO JY, ZHAO J, et al. Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus field strains in central China during 2010 to 2012 outbreaks[J]. Chinese Journal of Virology, 2013, 29(2):197-205. [27] 常铁城, 陈建飞, 冯力, 等. 2014年部分地区猪流行性腹泻病毒流行病学调查[J]. 中国预防兽医学报, 2016, 38(4):335-338. CHANG TC, CHEN JF, FENG L, et al. Epidemiological survey of porcine epidemic diarrhea virus in 2014[J]. Chinese Journal of Preventive Veterinary Medicine, 2016, 38(4):335-338. [28] 付萍. 中国部分地区猪流行性腹泻病毒分子流行病学调查[D]. 成都: 四川农业大学, 2022. FU P. Molecular epidemiological investigation of porcine epidemic diarrhea virus in some areas of China[D]. Chengdu: Sichuan Agricultural University, 2022. [29] 王鑫文, 吴静楠, 郭红瑞, 等. 猪流行性腹泻病毒云南省流行毒株S1基因序列分析[J]. 中国动物检疫, 2022, 39(9):8-25. WANG XW，WU JN，GUO HR, et al. Sequence analysis on S1 genes of PEDV Yunnan strains[J]. China Animal Health Inspection, 2022, 39(9):8-25. [30] CUI JT, QIAO H, HOU CY, et al. Characteristics of the spike and ORF3 genes of porcine epidemic diarrhea virus in Henan and Shanxi provinces of China[J]. Archives of Virology, 2020, 165(10):2323-2333.

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