China Swine Industry ›› 2021, Vol. 16 ›› Issue (4): 51-56.doi: 10.16174/j.issn.1673-4645.2021.04.011

• Swine Disease Prevention and Control • Previous Articles     Next Articles

Bioinformatics Analysis and Methylation Region Prediction of African Swine Fever Virus E165R Gene Promoter

ZHANG Yanbing1, ZHANG Shilei1, LIU Liangbo1, XIE Quanliang2, SUN Yanming1,*   

  1. 1College of Animal Science and Technology, Shihezi University, Shihezi 832003, China;
    2College of Life Science, Shihezi University, Shihezi 832003, China
  • Received:2021-05-30 Online:2021-08-25 Published:2021-09-16

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Abstract: ASF (African Swine Fever) is a severe, a cute and hemorrhagic infectious pigs' disease caused by ASFV (African Swine Fever Virus). The ASFV genome is double-stranded linear DNA (170~190 kb), which can encode more than 150~200 viral proteins. It has been reported that the E165R gene of ASFV was related to its replication, and the structure of the protein dUTPase encoded by E165R. This study aimed to investigate bioinformatics of the promoter of gene E165R, and then analyzed its promoter methylation region. The E165R promoter sequence of different ASFV strains was highly conserved. Through the prediction of promoters, such as activity analysis software and methylation software, we observed that the E165R promoter contains 6 transcription binding factors such as Sp1, c-Jun and C/EBPalp. Importantly, cg sites cover on binding sequences of Sp1, Oct-1 and C/EBPalp. We successfully predicted methylation region of E165R promoter. Methylation detection primer of E165R promoter was also designed. In this study, the ASFV E165R gene promoter was carried out for the first time from DNA methylation. It could be founded for the next step to study the influence of DNA methylation about ASFV replication.

Key words: ASFV, E165R, bioinformatics, promoter, DNA methylation

CLC Number: 

  • S828
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