长白猪和藏猪在不同日龄、种间的代谢物差异分析

doi:10.16174/j.issn.1673-4645.2024.02.003

摘要/Abstract

摘要： 通过对地方品种猪及外种猪的不同生长阶段代谢组学进行比较研究，以此了解地方品种猪与外种猪代谢组差异与耐粗饲性状的关系。采用UPLC-MS技术对7日龄和30日龄的长白猪和藏猪的粪便样品开展代谢组学研究，分析代谢物的变化情况，筛选差异代谢物的情况。结果显示，长白猪不同日龄代谢物VIP≥1 的总共有99种，其中38种代谢物的比例是上升的，另61种代谢物主要存在下降趋势；藏猪不同日龄代谢物VIP≥1的总共有90 种，其中31 种代谢物的比例是上升的，另59种代谢物存在下降趋势；7日龄的长白猪和藏猪代谢物对比发现VIP≥1的总共有59种，其中29种代谢物的比例主要是上升趋势，另30种是下降趋势；30日龄的长白猪和藏猪代谢物对比VIP≥1的总共有66种，其中32种代谢物的比例是上升的，另34种代谢物存在下降趋势。说明不同种间、日龄代谢物含量存在差异，其中同种不同日龄猪的代谢物差异最多，说明随着年龄的增长猪对粗纤维等粗饲料的消化代谢能力有所差异。通过对代谢通路的分析显示，主要存在差异的代谢通路为Metabolic pathways、ABC transporters，参与差异代谢通路的代谢物主要是有机酸、核苷酸、氨基酸等相关代谢物，说明藏猪和长白猪在耐粗饲的特性上与粗饲料对肠道的影响有一定的关联性。此研究为解释在代谢组学方面藏猪耐粗饲特性提供了一定的理论基础。

关键词: 藏猪, 长白猪, 代谢组学, 耐粗饲

中图分类号: S828；S821.61

李江凌, 张金灵, 陈晓晖, 王秋实, 赵素君, 刘锐, 吕学斌, 王宇萍, 龚建军, 何志平. 长白猪和藏猪在不同日龄、种间的代谢物差异分析[J]. 中国猪业, 2024, 19(2): 24-34.

参考文献

[1] 李文通, 严善英, 吴添文. 代谢组学在动物育种中的应用现状与前景展望[J]. 中国农业科技导报, 2022, 24(7):39-45. LI WT, YAN SY, WU TW. Applications and prospects of metabonomics in animal breeding[J]. Journal of Agricultural Science and Technology, 2022, 24(7):39-45. [2] 李江凌, 陈晓晖, 刘锐, 等. 藏猪耐粗饲特性及其生化机理研究[J]. 中国猪业, 2015, 10(2):70-72. LI JL, CHEN XH, LIU R, et al. Studies on the roughage tolerance of Tibetan pigs and its biochemical mechanism[J]. China Swine Industry, 2015, 10(2):70-72. [3] 谭碧娥, 伍树松, 贺建华, 等. 地方猪耐粗饲和肉质性状形成的微生物代谢机制[J]. 动物营养学报, 2020, 32(7):2941-2946. TAN BE, WU SS, HE JH, et al. Metabolic mechanism of coarse feeding tolerance and meat quality traits formation based on microbial metabolism in local pigs[J]. Chinese Journal of Animal Nutrition, 2020, 32(7):2941-2946. [4] 田菁, 王宇哲, 闫世雄, 等. 代谢组学技术发展及其在农业动植物研究中的应用[J]. 遗传, 2020, 42(5):452-465. TIAN J, WANG YZ, YAN SX, et al. Metabolomics technology and its applications in agriculturalanimal and plant research[J]. Hereditas, 2020, 42(5):452-465. [5] 温晓鹿. 以猪为模型研究高纤维或高脂肪日粮对肠道微生物及其代谢产物的影响[J]. 广东饲料, 2017, 26(7):51. WEN XL. Study of the effects of high-fiber or high-fat diets on intestinal microorganisms and their metabolites using pigs as a model[J]. Guangdong Feed, 2017, 26(7):51. [6] 王文娟, 孙冬岩, 孙笑非. 猪肠道微生物菌群及其与营养代谢的相互作用[J]. 饲料研究, 2023, 46(15):124-127. WANG WJ, SUN DY, SUN XF. Swine gut microbiota and its interaction with nutrient metabolism[J]. Feed Research, 2023, 46(15):124-127. [7] 文伟, 胡友军, 程皇座, 等. 有机酸对猪肠道菌群的调节作用及其抑菌机制研究进展[J]. 中国畜牧兽医, 2023, 50(8):3133-3141. WEN W, HU YJ, CHENG HZ, et al. Research progress of regulating effect of organic acidson intestinal flora of pigs and its bacteriostatic mechanism[J]. 2023, 50(8):3133-3141. [8] 张奇, 杨海天, 孔祥杰, 等. 日粮纤维对猪肠道微生物影响的研究进展[J]. 中国畜牧杂志, 2019, 55(2):11-14. ZHANG Q, YANG HT, KONG XJ, et al. Research progress on dietary fiber affecting intestinal microorganism of pigs[J]. Chinese Journal of Animal Science, 2019, 55(2):11-14. [9] 李开军, 蔡东森, 陈海军. 日粮纤维水平对猪肠道生理机能影响的研究进展[J]. 中国猪业, 2022, 17(5):43-46. LI KJ, CAI DS, CHEN HJ. Research advance on the effects of dietary fiber levels on the physiological functions of pig intestines[J]. China Swine Industry, 2022, 17(5):43-46. [10] 吴维达. 不同日粮纤维对生长猪血浆代谢组及肠道菌群影响的研究[D]. 北京: 中国农业科学院, 2016. WU WD. Study on the effect of dietary fiber on the plasma metabolomics and gut flora in growing pig[D]. Beijing: Chinese Academy of Agricultural Sciences, 2016. [11] 徐荣莹. 不同日粮纤维对生长猪肠道黏膜基因表达、菌群结构和代谢物的影响[D]. 南京: 南京农业大学, 2020. XU RY. Effects of the different dietary fibers on gut mucosal gene expression, microbiota composition and metabolites in growing pigs[D]. Nanjing: Nanjing Agricultural University, 2020. [12] 聂小燕, 林师庆, 何应沛, 等. 猪在不同阶段肠道微生物的变化及其对营养物质代谢的影响[J]. 黑龙江畜牧兽医, 2022(11):39-44. NIE XY, LIN SQ, HE YP, et al. Change of gut microbiota of pigs at different stages and its impact on host nutrition metabolism[J]. Heilongjiang Animal Science and Veterinery Medicine, 2022(11):39-44. [13] MAO GZ, WEI HL, CHEN PY, et al. Application of a high-density single nucleotide polymorphism genetic map in mapping quantitative trait loci of early-maturing traits in upland cotton[J]. Agronomy, 2023, 13(11). Doi: 10.3390/AGRONOMY13112716. [14] 郝小静, 吕慧敏, 张倩, 等. 肠道微生物代谢产物介导肠道免疫的研究进展[J]. 饲料研究, 2023, 581(23):168-173. HAO XJ, LV HM, ZHANG Q, et al. Research progress of intestinal microbial metabolites mediated intestinal immunity[J]. Feed Research, 2023, 581(23):168-173. [15] LARAIB U, RAHMANI AH, ALSAHLI MA, et al. Genetic profile of FOXO3 Single Nucleotide Polymorphism in colorectal cancer patients[J]. Oncology, 2023. doi: 10.1159/000533729. [16] YUAN J, ZHOU X, XU GQ, et al. Genetic diversity and population structure of Tongcheng pigs in China using whole-genome SNP chip[J]. Frontiers in Genetics, 2022, 13. doi: 10.3389/FGENE.2022.910521. [17] 赵金波, 刘盈序, 黄合特, 等. 地方猪肠道微生物高效利用日粮纤维的调控机制研究[J]. 中国饲料, 2023(15):1-7,38. ZHAO JB, LIU YX, HUANG HE, et al. Regulation mechanism of high-efficiency utilization of dietary fiber by intestinal microbiota in local pigs[J]. China Feed, 2023(15):1-7,38. [18] SALES MA, LARSON MJ, REITER ST, et al. Effects of bovine cytochrome P450 single-nucleotide polymorphism, forage type and body condition on production traits in cattle[J]. Journal of Animal Physiology and Animal Nutrition, 2012, 96(4):545-553. [19] 石宝明, 鲍嘉欣, 赵轩. 中国地方猪种肠道微生物功能发掘与利用的研究进展[J]. 动物营养学报, 2022, 34(10):6281-6290. SHI BM, BAO JX, ZHAO X. Research progress on exploitation and utilization of intestinal microbial function in Chinese native pigs[J]. Chinese Journal of Animal Nutrition, 2022, 34(10):6281-6290. [20] 谭占坤, 商振达, 褚瑰燕, 等. 藏猪对饲粮纤维的消化及其与肠道微生物的相关性研究[J]. 畜牧兽医学报, 2022, 53(9):3063-3078. TAN ZK, SHANG ZD, CHU GY, et al. Study on dietary fibre digestion and Its correlation with intestinal microbiome of Tibetan pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9):3063-3078. [21] HAZARIKA D, AMONGE TK, BORPUZARI RN, et al. Productive Performance of Crossbred (Hampshire x Assam local) Pigs Maintained onAzolla Protein Substituted Feed[J]. The Indian Veterinary Journal, 2015, 92(10):79-81.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed