蕨麻猪产仔性状全基因组关联分析

doi:10.16174/j.issn.1673-4645.2024.02.005

摘要/Abstract

摘要： 本研究旨在挖掘影响蕨麻猪总产仔数（Total number born, TNB）和产活仔数（Number born alive, NBA）的候选基因，为蕨麻猪高繁殖力基因组选育提供参考数据。采集140头蕨麻猪耳组织样品，提取DNA，利用50K 液相芯片进行SNPs 检测。分别采用广义线性模型和混合线性模型对140 头蕨麻猪个体的SNPs 与TNB 和NBA 进行全基因组关联分析（Genome-wide association studies, GWAS），筛选影响该群体繁殖性状的候选基因。结果显示院针对TNB 和NBA等2 个性状，广义线性模型分别检测到14 个和1 个显著的SNPs 位点。混合线性模型检测到TNB 有2 个显著的SNPs 位点；未检测到显著影响NBA 的SNP。筛选ZCCHC24、ENSSSCG00000046125 和TENM1 作为影响TNB 的重要候选基因，ZCCHC24 作为影响NBA 的重要候选基因。

中图分类号: S828；S813

张丰霞，杨德威，崔茂盛，李千军，闫峻，旦智草. 蕨麻猪产仔性状全基因组关联分析[J]. 中国猪业, 2024, 19(2): 43-51.

参考文献

[1] 张珍, 翟丹云, 高晓辉. 地理标志产品迭部蕨麻猪肉的标准制定及产业发展研究[J]. 甘肃畜牧兽医, 2020, 50(7):23-26. ZHANG Z, ZHAI DY, GAO XH. Research on standardization and industrial development of Diebu Juema pork, a geographical indication product[J]. Gansu Animal Husbandry and Veterinary, 2020, 50(7):23-26. [2] 麻建萍. 蕨麻猪养殖现状及发展建议[J]. 国外畜牧学（猪与禽）, 2020, 40(1):2. MA JP. Current situation and development suggestions of Juema pig breeding[J]. Animal Science Abroad (Pigs and Poultry), 2020, 40(1):2. [3] 严肃才. 迭部县蕨麻猪养殖现状调研报告 [J]. 中国畜禽种业, 2020, 16(12): 11-12. YAN SC. Investigation report on the current situation of Juema pig breeding in Diebu county[J]. China Livestock and Poultry Breeding, 2020, 16(12): 11-12. [4] 蒋志勇, 薛瑞林. 甘南蕨麻猪的养殖现状及发展建议 [J]. 猪业科学, 2023, 40(5): 120-123. JIANG ZY, XUE RL. Breeding status and development suggestions of Gannan Juema pig breeding[J]. Swine Industry Science, 2023, 40(5): 120-123. [5] 杨秀花. 迭部蕨麻猪饲养管理技术要点 [J]. 国外畜牧学(猪与禽), 2023, 43(1): 44-46. YANG XH. Key points of breeding and management technology of Diebu Juema pig[J]. Animal Science Abroad (Pigs and Poultry), 2023, 43(1): 44-46. [6] CHEN P, BAAS T J, MABRY J W, et al. Genetic parameters and trends for litter traits in U.S. Yorkshire, Duroc, Hampshire, and Landrace pigs [J]. Journal of Animal Science, 2003, 81(1): 46-53. [7] HOLM B, BAKKEN M, VANGEN O, et al. Genetic analysis of age at first service, return rate, litter size, and weaning-to-first service interval of gilts and sows[J]. Journal of Animal Science, 2005, 83(1): 41-48. [8] GODDARD ME, HAYES BJ. Mapping genes for complex traits in domestic animals and their use in breeding programmes[J]. Nature Reviews Genetics, 2009, 10(6):381-391. [9] ZHANG XD, ZHU HY, Zhou J, et al. Relationship between polymorphisms in exon 10 of FSHR gene and litter size in swine[J]. Genetics and Molecular Research, 2015, 14(3): 8252-8261. [10] KARIUKI CM, BRASCAMP EW, KOMEN H, et al. Economic evaluation of progeny-testing and genomic selection schemes for small-sized nucleus dairy cattle breeding programs in developing countries[J]. Journal of Dairy Science, 2017, 100(3):2258-2268. [11] SONG H, ZHANG J, JIANG Y, et al. Genomic prediction for growth and reproduction traits in pig using an admixed reference population[J]. Journal of Animal Science, 2017, 95(8):3415-3424. [12] VARONA L, LEGARRA A, HERRING W, et al. Genomic selection models for directional dominance: an example for litter size in pigs[J]. Genetics Selection Evolution, 2018, 50(1):1. [13] WOLC A, ZHAO HH, ARANGO J, et al. Response and inbreeding from a genomic selection experiment in layer chicken [J]. Genetics Selection Evolution, 2015, 47(1):59. [14] 蔡薇, 罗才玉, 项云, 等. 基于50K SNP芯片技术对金华猪和嵊县花猪繁殖性状的全基因组关联分析[J]. 中国畜牧杂志, 2020, 56(7):52-58. CAI W, LUO CY, XIANG Y, et al. Genome-wide association analysis of reproductive traits of Jinhua pig and Shengxian pig based on 50K SNP chip technology[J]. Chinese Journal of Animal Science, 2020, 56(7):52-58. [15] 宋志芳. 利用GWAS对深县猪繁殖性状的研究[D] 保定: 河北农业大学, 2018. SONG ZF. Study on reproductive traits of Shenxian pig in using GWAS[D] Baoding: Hebei Agricultural University, 2018. [16] 莫家远, 高九昱, 奉玲丽, 等. 巴马香猪产活仔数性状全基因组关联分析[J]. 中国畜牧兽医, 2020, 47(12):3965-3975. MO JY, GAO JY, FENG LL, et al. Genome-wide association study on alive litter size trait in Bama Xiang pigs[J]. China Animal Husbandry & Veterinary Medicine, 2020, 47(12):3965-6975. [17] 陈俊峰, 王璟, 张家庆, 等. 全基因组关联分析筛选淮南猪繁殖性状候选基因[J]. 中国畜牧杂志, 2023, 59(7):111-114. CHEN JF, WANG J, ZHANG JQ, et al. Screening of candidate genes for reproductive traits in Huainan pigs by genome-wide association analysis[J]. Chinese Journal of Animal Science, 2023, 59(7):111-114. [18] 朱吉, 任慧波, 吴发平, 等. 广益黑猪繁殖性状的全基因组关联分析 [J]. 湖南农业大学学报(自然科学版), 2023, 49(4):453-460. ZHU J, REN HB, WU FP, et al. Genome-wide association analysis of reproductive traits in Guangyi black pigs[J]. Journal of Hunan Agricultural University (Natural Sciences), 2023, 49(4):453-460. [19] BENJAMINI Y, HOCHBERG Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing[J]. Journal of the Royal Statistical Society Series B: Methodological, 1995, 57(1):289-300. [20] WELLER JI, SONG JZ, HEYEN DW, et al. A new approach to the problem of multiple comparisons in the genetic dissection of complex traits[J]. Genetics, 1998, 150(4):1699-1706. [21] 陶虎, 张元, 杨娟, 等. 山羊环状RNA circ＿ZCCHC24表达载体的构建及对颗粒细胞增殖的影响[J]. 中国畜牧杂志, 2019, 55(10):116-21. TAO H, ZHANG Y, YANG J, et al. Construction of circ_ZCCHC24 expression vector of Goat Circ_ZCCHC24 and its effect on proliferation of granulosa cells[J]. Chinese Journal of Animal Science, 2019, 55(10):116-121. [22] VITORINO M, CORREIA E, SERRALHEIRO AR, et al. Expression pattern of zcchc24 during early Xenopus development[J]. International Journal of Developmental Biology, 2014, 58(1):45-50. [23] ZHU Y, LUO M, HUANG H, et al. HOXA10, EMX2 and TENM1 expression in the mid-secretory endometrium of infertile women with a Mullerian duct anomaly[J]. Reproductive Biomedicine Online, 2016, 32(4):388-393. [24] 李昆谕, 刘玉芳, 陶林, 等. 山羊ESR1?TENM1和THEM4多态性及其与繁殖性状相关性研究[J]. 安徽农业科学, 2023, 51(1):79-84. LI KY, LIU YF, TAO L, et al. Study on the polymorphism of ESR1, TENM1 and THEM4 and their correlation with the reproductive traits of goats[J]. Journal of Anhui Agricultural Sciences, 2023, 51(1):79-84. [25] BOUAZIZ M, AMBROISE C, GUEDJ M. Accounting for population stratification in practice: a comparison of the main strategies dedicated to genome-wide association studies[J]. PLoS One, 2011, 6(12):e28845. doi: 10.1371/journal.pone.0028845.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed