猪圆环病毒3型快速检测方法研究进展

doi:10.16174/j.issn.1673-4645.2023.05.018

摘要/Abstract

摘要： 猪圆环病毒3型可引起猪的皮疹、呼吸系统和泌尿系统功能障碍等。快速、准确地检测PCV3对于保障猪的健康和生产具有重要意义。本文综述了当前国内外已报道的PCV3快速检测方法，主要包括免疫学检测方法(酶联免疫吸附试验、免疫层析技术)、基于PCR的方法(常规PCR、套式PCR、多重PCR、荧光定量PCR、数字PCR及其他基于PCR的技术)、等温扩增技术(环介导等温扩增、重组酶介导等温核酸扩增、重组酶聚合酶扩增、酶促重组等温扩增以及聚合酶螺旋反应)、原位杂交技术以及宏基因组测序技术。本文还初步探讨了不同方法的原理、特性及优缺点，展望了未来PCV3快速检测方法的发展方向，以期为快速、精准地检测及科学防控PCV3提供参考。

关键词: 生猪, 猪圆环病毒3型, 免疫学技术, PCR技术, 等温扩增技术

庄林林, 陈欣雅, 徐佳豪, 宋春雷, 申秋平. 猪圆环病毒3型快速检测方法研究进展[J]. 中国猪业, 2023, 18(5): 77-84.

参考文献

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