中国猪业 ›› 2023, Vol. 18 ›› Issue (5): 70-73.doi: 10.16174/j.issn.1673-4645.2023.05.016

• 猪病防控 • 上一篇    

金银花源miR2911靶向非洲猪瘟病毒基因区域分析

杜非凡,陈星宇,李硕,张昊,常怡悦,张自瑞,闫卫疆,肖非,孙雪梅,盛金良,孙延鸣,张彦兵   

  • 出版日期:2023-10-25 发布日期:2023-10-31

  • Online:2023-10-25 Published:2023-10-31

摘要: 金银花源miR2911是一种高丰度、性质稳定的抗病毒分子,在抗流感病毒感染、SARS-CoV-2感染中具有一定效果。然而,对于miR2911靶向ASFV基因区域的研究却鲜有报道。由此,本研究旨在分析miR2911靶向ASFV的基因区域,从而进一步分析miR2911靶点在不同流行毒株中的保守性。利用miRanda软件成功预测了miR2911在ASFV基因组中存在的多个靶点,且靶向ASFV基因区域保守性很高。为后续深入研究miR2911在抗猪源病毒感染机理方面提供理论依据。

关键词: 猪病;miR2911;病毒基因;非洲猪瘟;金银花

[1] Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function[J]. Cell, 2004, 116(2):281-297. [2] Ambros V. The functions of animal microRNAs[J]. Nature, 2004, 431(7006):350-355. [3] Lee Y, Kim M, Han J, et al. MicroRNA genes are transcribed by RNA polymerase Ⅱ[J]. The EMBO Journal, 2004, 23(20):4051-4060. [4] Gregory RI, Chendrimada TP, Shiekhattar R. MicroRNA biogenesis: isolation and characterization of the microprocess or complex[J]. Methods in Molecular Biology, 2006, 342:33-47. [5] Lund E, Dahlberg JE. Substrate selectivity of exportin 5 and Dicer in the biogenesis of microRNAs[C]//. In: 71st Cold Spring Harbor Symposium on Quantitative Biology, Cold Spring, NY, 2006. doi: 10.1101/sqb.2006.71.050. [6] Murchison EP, Hannon GJ. MiRNAs on the move: miRNA biogenesis and the RNAi machinery [J]. Current Opinion in Cell Biology, 2004, 16(3):223-229. [7] Lund E, Güttinger S, Calado A, et al. Nuclear export of microRNA precursors[J]. Science, 2004, 303(5654):95-98. [8] Yi R, Qin Y, Macara IG, et al. Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs [J]. Genes Development, 2003, 17(24):3011-3016. [9] Almeida MI, Reis RM, Calin GA. MicroRNA history: discovery, recent applications, and next frontiers[J]. Mutation Research,2011, 717(1-2):1-8. [10] Hayes J, Peruzzi PP, Lawler S. MicroRNAs in cancer: biomarkers, functions and therapy[J]. Trends in Molecular Medicine, 2014, 20(8):460-469. [11] Yang MS, Law FC, Wong RN, et al. Interaction between oseltamivir and herbal medicines used for treating avian influenza[J]. Hong Kong Medical Journal, 2012, 18(Suppl 6):34-36. [12] Zhou Z, Li X, Liu J, et al. Honeysuckle-encoded atypical microRNA2911 directly targets influenzaA viruses[J]. Cell Research,2015, 25(1):39-49. [13] Zhou LK, Zhou Z, Jiang XM, et al. Absorbed plant MIR2911 in honeysuckle decoction inhibits SARS-CoV-2 replication and accelerates the negative conversion of infected patients[J]. Cell Discovery, 2020, 6(1):54. [14] Wang N, Zhao D, Wang J, et al. Architecture of african swine fever virus and implications for viral assembly [J]. Science, 2019, 366(6465):640-644. [15] Blome S, Gabriel C, Beer M. Pathogenesis of African swine fever in domestic pigs and European wild boar [J]. Virus Research, 2013, 173(1):122-130.
No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!