一株母猪产道益生性粪肠球菌的分离鉴定

doi:10.16174/j.issn.1673-4645.2021.03.010

中国猪业 ›› 2021, Vol. 16 ›› Issue (3): 44-50.doi: 10.16174/j.issn.1673-4645.2021.03.010

一株母猪产道益生性粪肠球菌的分离鉴定

汪溪¹, 汪惠琳¹, 曹刚¹, 黎佳杰¹, 陈松昌², 冯祥瑞^3,*

¹江西农业大学动物科学技术学院,江西南昌 330045;
²南昌金牧动物保健服务有限公司,江西南昌 330013;
³永丰县农业综合行政执法大队,江西永丰 331500

收稿日期:2021-05-07 出版日期:2021-06-25 发布日期:2021-07-15
通讯作者: ^*冯祥瑞（1972-）,男,江西永丰人,从事兽医疫病防控技术研究,E-mail：jxyfnyzfdd@163.com
作者简介:汪溪（1999-）,女,江西南昌人,本科在读,研究方向为预防兽医专业,E-mail：1837357710@qq.com
基金资助:
江西农业大学大学生创新创业项目（158）

Isolation and Identification of Enterococcus Faecalis Beneficial to the Birth Canal of Sow

WANG Xi¹, WANG Huilin¹, CAO Gang¹, LI Jiajie¹, CHEN Songchang², FENG Xiangrui^3,*

¹College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China;
²Nanchang Jinmu Animal Health Service Co. Ltd, Nanchang 330045, China;
³Yongfeng County Agricultural Comprehensive Administrative Law Enforcement Brigade, Yongfeng 330045, China

Received:2021-05-07 Online:2021-06-25 Published:2021-07-15

摘要/Abstract

摘要： 产道作为母猪生殖系统的重要器官组织,与繁殖性能密切相关。本试验无菌采集江西省某养殖场窝产14头母猪产道中段阴道分泌物进行传统细菌分离培养,初步筛选出4株细菌,挑选其中一株通过生化反应和16S rDNA序列分析方法进行鉴定,同时进行抗生素抵抗能力试验和牛津杯法体外抑制试验。生化鉴定结果表明该菌可降解果糖、乳糖、半乳糖等,该菌代谢产物与葡萄糖酸盐无反应,葡萄糖产气试验说明该菌产酸不产气。分离菌株通过NCBI同源性比对为粪肠球菌（登录号EU794735.1）。分离菌对青霉素、头孢哌酮等高度敏感,对红霉素、庆大霉素等耐药。体外抑制试验中分离菌对大肠杆菌k88和变形杆菌具有良好的抑制效果,可以作为候选益生菌株进行深入研究。这为今后益生菌代替抗生素及产道菌群移植防治母猪产道炎症提供理论基础。

关键词: 母猪, 粪肠球菌, 产道, 分离鉴定, 体外抑菌

Abstract: As an important organ of sow reproductive system, the birth canal was closely related to reproductive performance. In order to obtain microorganism resources in the birth canal of sows, the vaginal secretions of 14 sows from a breeding farm in Jiangxi province were aseptically collected for traditional bacterial isolation and culture. 4 strains of bacteria were screened preliminarily, and one of them was identified by biochemical reaction and 16S rDNA sequence analysis. At the same time, antibiotic resistance test and Oxford cup method in vitro inhibition test were carried out. In biochemical identification, fructose, lactose, galactose and so on were positive, gluconate was negative, glucose gas production test indicated that the strain was acid producing but not gas producing. The homology ratio of the isolates on NCBI was Enterococcus faecalis (accession number EU794735.1). The isolates were highly sensitive to penicillin and cefoperazone, and resistant to erythromycin and gentamicin. In vitro inhibition test, the isolates showed good inhibitory effect on Escherichia coli K88 and Proteus, which could be used as candidate probiotics for further study. It could provided a theoretical basis for probiotics to replace antibiotics and will transplant of birth canal bacteria to prevent and control birth canal inflammation of sows in the future.

Key words: sow, enterococcus faecalis, vaginal, in vitro bac

中图分类号: S828;S852.61

汪溪, 汪惠琳, 曹刚, 黎佳杰, 陈松昌, 冯祥瑞. 一株母猪产道益生性粪肠球菌的分离鉴定[J]. 中国猪业, 2021, 16(3): 44-50.

WANG Xi, WANG Huilin, CAO Gang, LI Jiajie, CHEN Songchang, FENG Xiangrui. Isolation and Identification of Enterococcus Faecalis Beneficial to the Birth Canal of Sow[J]. China Swine Industry, 2021, 16(3): 44-50.

参考文献

[1] 田丰松. 治疗奶牛子宫内膜炎益生乳酸杆菌筛选及临床效果研究[D]. 长春: 吉林农业大学, 2017.
[2] 许苏容, 宗利丽, 刘木彪, 等. Illumina测序16S rRNA标签法分析细菌性阴道病患者阴道菌群多样性[J]. 南方医科大学学报, 2013, 33(5):672-677.
[3] Kazuaki Y, Nobuo M, Kazumasa F, et al.Effects of pelvic organ prolapse ring pessary therapy on intravaginal microbial flora[J]. International Urogynecology Journal, 2016, 27(2):219-227.
[4] Otero MC, Nader-Macías ME.Inhibition of Staphylococcus aureus by H₂O₂-producing Lactobacillus gasseri isolated from the vaginal tract of cattle[J]. Animal Reproduction Science, 2006, 96(1-2):35-46.
[5] Wagener K, Prunner I, Pothmann H, et al.Diversity and health status specific fluctuations of intrauterine microbial communities in postpartum dairy cows[J]. Veterinary Microbiology, 2015, 175(2-4):286-293.
[6] 王亚萍, 谢幸. 阴道菌群在HPV感染和宫颈病变发生发展中的作用[J]. 现代妇产科进展, 2018, 27(11):873-874.
[7] 万倩, 李启明, 吴华星, 等. 传统发酵食品中乳酸菌的安全性评估[J]. 现代食品科技, 2021, 37(6):276-286.
[8] Wang AR, Ran C, Wang YB, et al.Use of probiotics in aquaculture of China—a review of the past decade[J]. Fish and Shellfish Immunology, 2019, 86(3):734-755.
[9] 郭淑敏, 周秀丽, 朱玲. 阴道用乳酸菌制剂辅助治疗RVVC的Meta分析[J]. 中国微生态学杂志, 2018, 30(11):1317-1325.
[10] Santacroce, Charitos, Bottalico. A successful history: probiotics and their potential as antimicrobials[J]. Expert Review of Anti-infective Therapy, 2019, 17(8):635-645.
[11] 呙会会. 神农架金丝猴源肠道益生菌的筛选和鉴定[D]. 武汉: 华中农业大学, 2014.
[12] 廖延雄. 《伯杰氏鉴定细菌学手册》与《伯杰氏分类细菌学手册》[J]. 微生物学通报, 1992(4):249.
[13] 王佳慧, 姜仲文, 满永恒, 等. 粪肠球菌BN6株的体外益生特性评价[J]. 中国兽医科学, 2021, 51(3):337-342.
[14] 石春卫, 谢静, 杨桂连, 等. 猪源粪肠球菌的分离与鉴定[J]. 动物医学进展, 2020, 41(9):123-127.
[15] 徐帅帅, 李安章, 朱家杭, 等. 规模化养殖条件下生猪肠道微生物资源分离和鉴定[J]. 生物资源, 2020, 42(5):549-556.
[16] 李佳徽, 王明阳, 田相利, 等. 三种不同乳酸菌对凡纳滨对虾生长、抗病力及肠道菌群结构的影响[J]. 中国海洋大学学报(自然科学版), 2021, 51(4):44-54.
[17] 席晓霞. 初生婴儿肠道菌群与母体各部位菌群相关性研究[D]. 呼和浩特: 内蒙古农业大学, 2017.

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一株母猪产道益生性粪肠球菌的分离鉴定

Isolation and Identification of Enterococcus Faecalis Beneficial to the Birth Canal of Sow

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