Abstract: In order to understand the antibodies against common infectious diseases in a large-scaled pig farm in northern Xinjiang, so as to find the potential disease risk of the pig farm in time. In this study, indirect enzyme-linked immunosorbent assay (ELISA) was used to detect the antibodies against classical swine fever virus(CSFV), porcine reproductive and respiratory syndrome virus(PRRSV), porcine circovirus type 2 (PCV2), porcine foot-and-mouth disease virus type O(FMDV-O), and porcine pseudorabies virus (PRV)gB and gE protein in the pig farm. The test results showed that the overall antibody positive rates of CSFV, PRRSV, PCV-2, FMDV-O, PRV-gB protein, and PRV-gE protein in the field were 83.57%, 90.56%, 90.29%, 71.86%, 82.84% and 18.29%, respectively; antibody levels were uneven among different types of pig herds, the positive rate of PRRSV antibody in breeding herd was only 52.63%, the positive rate of CSFV antibody in nursery herd was only 44.12%, and the positive rate of PRV-gB antibody in fattening herd was only 30.00%, the positive rate of PRV-gE antibody in breeding boars was as high as 30.00%, and the positive rate of FMDV-O antibody in fattening pigs was only 6.25%. Based on the above test results, it provided a reference for the formulation and optimization of the immunization program of this farm and achieving the purpose of effective control and gradual purification of the epidemic.

Key words: antibody detection, enzyme-linked immunosorbent assay, immunization schedule