Abstract: As an important organ of sow reproductive system, the birth canal was closely related to reproductive performance. In order to obtain microorganism resources in the birth canal of sows, the vaginal secretions of 14 sows from a breeding farm in Jiangxi province were aseptically collected for traditional bacterial isolation and culture. 4 strains of bacteria were screened preliminarily, and one of them was identified by biochemical reaction and 16S rDNA sequence analysis. At the same time, antibiotic resistance test and Oxford cup method in vitro inhibition test were carried out. In biochemical identification, fructose, lactose, galactose and so on were positive, gluconate was negative, glucose gas production test indicated that the strain was acid producing but not gas producing. The homology ratio of the isolates on NCBI was Enterococcus faecalis (accession number EU794735.1). The isolates were highly sensitive to penicillin and cefoperazone, and resistant to erythromycin and gentamicin. In vitro inhibition test, the isolates showed good inhibitory effect on Escherichia coli K88 and Proteus, which could be used as candidate probiotics for further study. It could provided a theoretical basis for probiotics to replace antibiotics and will transplant of birth canal bacteria to prevent and control birth canal inflammation of sows in the future.

Key words: sow, enterococcus faecalis, vaginal, in vitro bac